Validation Data Gallery
Tested Applications
| Positive WB detected in | Daudi cells, Ramos cells, U-251 cells |
| Positive IP detected in | Daudi cells, U-251 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31489-1-AP targets SEL1L3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34856 Product name: Recombinant human SEL1L3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-236 aa of BC060867 Sequence: KYSGRDWNVKWEENLLHAVAKNYTLLQTIPPFERPFKDHQVCLEWNMGYIWNLRANRIPQCPLENDVVALLGFPYASSGENTGIVKKFPRFRNRELEATRRQRMDYPVFTVSLWLYLLHYCKANLCGILYFVDSNEMYGTPSVFLTEEGYLHIQMHLVKGEDLAVKTKFIIPLKEWFRLDISFNGGQIVVTTSIGQDLKSYHNQTISFRE 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA0746 protein |
| Observed molecular weight | 129 kDa |
| GenBank accession number | BC060867 |
| Gene Symbol | KIAA0746 |
| Gene ID (NCBI) | 23231 |
| RRID | AB_3742437 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q68CR1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SEL1L3 is also named suppressor of lin-12-like protein 3. SEL1L3, a protein with multiple glycosylation sites, was identified as the BCR target in 3/20 PVRL cases. SEL1L3 induces proliferation and BCR pathway activation in aggressive lymphoma cell lines. Moreover, SEL1L3 conjugated to a toxin killed exclusively lymphoma cells with respective BCR-reactivity (PMID: 38671086).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SEL1L3 antibody 31489-1-AP | Download protocol |
| IP protocol for SEL1L3 antibody 31489-1-AP | Download protocol |
| WB protocol for SEL1L3 antibody 31489-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |