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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC22B Recombinant monoclonal antibody (85037-5-RR)

    WB analysis using 85037-5-RR (same clone as 85037-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85037-5-RR (SEC22B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85037-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SEC22B Recombinant monoclonal antibody (85037-5-RR)

    IHC staining of mouse kidney using 85037-5-RR (same clone as 85037-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85037-5-RR (SEC22B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85037-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SEC22B Recombinant monoclonal antibody (85037-5-RR)

    IHC staining of mouse kidney using 85037-5-RR (same clone as 85037-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85037-5-RR (SEC22B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85037-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SEC22B Recombinant monoclonal antibody (85037-5-RR)

    IHC staining of mouse liver using 85037-5-RR (same clone as 85037-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85037-5-RR (SEC22B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85037-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SEC22B Recombinant monoclonal antibody (85037-5-RR)

    IHC staining of mouse liver using 85037-5-RR (same clone as 85037-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85037-5-RR (SEC22B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85037-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using SEC22B Recombinant monoclonal antibody (85037-5-RR)

    FC experiment of SH-SY5Y using 85037-5-RR (same clone as 85037-5-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SEC22B Recombinant antibody (85037-5-RR, Clone:242659B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 85037-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85037-5-RR

    Affinity and Kinetic Characterization of 85037-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85037-5-RR against Human SEC22B were performed. The affinity constant is 2.07 nM.

SEC22B Recombinant monoclonal antibody, PBS Only

SEC22B Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 85037-5-PBS
Clone No.242659B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

242659B3, ER-Golgi SNARE of 24 kDa, ERS24, ERS-24, SEC22 vesicle-trafficking protein homolog B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85037-5-PBS targets SEC22B in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Tested Reactivity human, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag12981

Product name: Recombinant human SEC22B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-215 aa of BC001364

Sequence: VLLTMIARVADGLPLAASMQEDEQSGRDLQQYQSQAKQLFRKLNEQSPTRCTLEAGAMTFHYIIEQGVCYLVLCEAAFPKKLAFAYLEDLHSEFDEQHGKKVPTVSRPYSFIEFDTFIQKTKKLYIDSRARRNLGSINTELQDVQRIMVANIEEVLQRGEALSALDSKANNLSSLSKKYRQDAKYLNMRSTYAKLAAVAVFFIMLIVYVRFWWL

相同性解析による交差性が予測される生物種
Full Name SEC22 vesicle trafficking protein homolog B (S. cerevisiae)
Calculated molecular weight 25 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC001364
Gene Symbol SEC22B
Gene ID (NCBI) 9554
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO75396
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) proteins are critical components of the membrane fusion machinery (PMID: 20163564). SEC22B is a SNARE involved in endoplasmic reticulum (ER)/Golgi membrane trafficking. SEC22B localizes to the ER-Golgi intermediate compartment (ERGIC) and has been identified as a regulator of antigen crosspresentation (PMID: 22153078).

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