Validation Data Gallery
Tested Applications
| Positive FC detected in | Transfected HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CLV510-98399 targets SDC2 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg2700 Product name: Recombinant Mouse SDC2 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 19-145 aa of NM_008304.2 Sequence: ETRTELTSDKDMYLDNSSIEEASGVYPIDDDDYSSASGSGADEDIESPVLTTSQLIPRIPLTSAASPKVETMTLKTQSITPAQTESPEETDKEEVDISEAEEKLGPAIKSTDVYTEKHSDNLFKRTE 相同性解析による交差性が予測される生物種 |
| Full Name | syndecan 2 |
| Calculated molecular weight | 22 kDa |
| GenBank accession number | NM_008304.2 |
| Gene Symbol | SDC2 |
| Gene ID (NCBI) | 15529 |
| RRID | AB_3749169 |
| Conjugate | CoraLux™ Violet 510 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 410 nm / 501 nm |
| 激发激光 | Violet Laser (405 nm) |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P43407 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
SDC2, containing cell surface heparan sulphate proteoglycan, functions as an integral membrane protein and participates in cell proliferation, cell migration, cell adhesion and signal transduction. It has been reported that the expression level of SDC2 will be up-regulated under hypoxia condition which can induce increased release of cytokines and chemokines. Besides, methylated SDC2 can be used as a potential biomarker for early diagnosis of colon cancer. 67088-1-Ig antibody detects the 19 and 22 kDa SDC2 isoforms as well as the 48 kDa dimeric protein in SDS-PAGE.(PMID: 18803305, 23297089, 30022330, 29945573)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CoraLux™ Violet 510 SDC2 antibody CLV510-98399 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |