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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCO1 Polyclonal antibody (12614-1-AP)

    WB analysis using 12614-1-AP (same clone as 12614-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using SCO1 Polyclonal antibody (12614-1-AP)

    WB analysis of human heart using 12614-1-AP (same clone as 12614-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SCO1 Polyclonal antibody (12614-1-AP)

    WB analysis of HepG2 using 12614-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using SCO1 Polyclonal antibody (12614-1-AP)

    WB analysis of LNCaP using 12614-1-AP (same clone as 12614-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using SCO1 Polyclonal antibody (12614-1-AP)

    WB analysis of human placenta using 12614-1-AP (same clone as 12614-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using SCO1 Polyclonal antibody (12614-1-AP)

    IP experiment of HepG2 using 12614-1-AP (same clone as 12614-1-PBS)

    IP result of anti-SCO1 (IP:12614-1-AP, 4ug; Detection:12614-1-AP 1:1000) with HepG2 cells lysate 1600 ug. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SCO1 Polyclonal antibody (12614-1-AP)

    IHC staining of human stomach cancer using 12614-1-AP (same clone as 12614-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12614-1-AP (SCO1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

SCO1 Polyclonal antibody, PBS Only

SCO1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 12614-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

SCOD1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12614-1-PBS targets SCO1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3269

Product name: Recombinant human SCO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC015504

Sequence: MAMLVLVPGRVMRPLGGQLWRFLPRGLEFWGPAEGTARVLLRQFCARQAEAWRASGRPGYCLGTRPLSTARPPPPWSQKGPGDSTRPSKPGPVSWKSLAITFAIGGALLAGMKHVKKEKAEKLEKERQRHIGKPLLGGPFSLTTHTGERKTDKDYLGQWLLIYFGFTHCPDVCPEELEKMIQVVDEIDSITTLPDLTPLFISIDPERDTKEAIANYVKEFSPKLVGLTGTREEVDQVARAYRVYYSPGPKDEDEDYIVDHTIIMYLIGPDGEFLDYFGQNKRKGEIAASIATHMRPYRKKS

相同性解析による交差性が予測される生物種
Full Name SCO cytochrome oxidase deficient homolog 1 (yeast)
Calculated molecular weight 301 aa, 34 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC015504
Gene Symbol SCO1
Gene ID (NCBI) 6341
RRIDAB_2184641
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75880
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Human SCO1 and SCO2 are metallochaperones that are essential for the assembly of the catalytic core of cytochrome c oxidase (COX). SCO1 appears to be involved in the folding and assembly of cytochrome oxidase subunit II, and possibly of other proteins of this subcompartment. It is a 33 kDa protein which displays limited homology to members of the DnaJ family(PMID:14731768). It is also named as SCOD1 and belongs to the SCO1/2 family. It can exsit as a homodimer(PMID:16735468). Defects in SCO1 are a cause of mitochondrial complex IV deficiency (MT-C4D).

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