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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SCO1 Polyclonal antibody

SCO1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 12614-1-AP

Synonyms

SCO1, SCOD1

Validation Data Gallery

Applications:
  • WB analysis using 12614-1-AP

    WB analysis using 12614-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 12614-1-AP

    WB analysis of HepG2 using 12614-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 12614-1-AP

    WB analysis of LNCaP using 12614-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 12614-1-AP

    WB analysis of human placenta using 12614-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 12614-1-AP

    WB analysis of human heart using 12614-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, LNCaP cells, human heart tissue, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

12614-1-AP targets SCO1 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCO1 fusion protein Ag3269 相同性解析による交差性が予測される生物種
Full Name SCO cytochrome oxidase deficient homolog 1 (yeast)
Calculated molecular weight 301 aa, 34 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC015504
Gene symbol SCO1
Gene ID (NCBI) 6341
RRIDAB_2184641
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human SCO1 and SCO2 are metallochaperones that are essential for the assembly of the catalytic core of cytochrome c oxidase (COX). SCO1 appears to be involved in the folding and assembly of cytochrome oxidase subunit II, and possibly of other proteins of this subcompartment. It is a 33 kDa protein which displays limited homology to members of the DnaJ family(PMID:14731768). It is also named as SCOD1 and belongs to the SCO1/2 family. It can exsit as a homodimer(PMID:16735468). Defects in SCO1 are a cause of mitochondrial complex IV deficiency (MT-C4D).

Protocols

Product Specific Protocols
WB protocol for SCO1 antibody 12614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,IP

Cell Rep

Downregulation of hepatic ceruloplasmin ameliorates NAFLD via SCO1-AMPK-LKB1 complex

Authors - Liping Xie
  • KD Validated
View Article