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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCML1 Polyclonal antibody (15093-1-AP)

    WB analysis using 15093-1-AP (same clone as 15093-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 15093-1-AP (SCML1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15093-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SCML1 Polyclonal antibody (15093-1-AP)

    IHC staining of mouse testis using 15093-1-AP (same clone as 15093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15093-1-AP (SCML1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15093-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SCML1 Polyclonal antibody (15093-1-AP)

    IHC staining of mouse testis using 15093-1-AP (same clone as 15093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15093-1-AP (SCML1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15093-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SCML1 Polyclonal antibody (15093-1-AP)

    IF Staining of A549 using 15093-1-AP (same clone as 15093-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SCML1 antibody (15093-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 15093-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using SCML1 Polyclonal antibody (15093-1-AP)

    FC experiment of A549 using 15093-1-AP (same clone as 15093-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SCML1 Polyclonal antibody (15093-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 15093-1-PBS in a different storage buffer formulation.

SCML1 Polyclonal antibody, PBS Only

SCML1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 15093-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Sex comb on midleg-like protein 1, SCML 1, Gm47, Gm1792

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15093-1-PBS targets SCML1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5107

Product name: Recombinant human SCML1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-302 aa of BC026159

Sequence: MMSNSSSEIDVQEPNIVSDASCNTEEQLKTVDDVLIHCQVIYDALQNLDKKIDVIRRKVSKIQRFHARSLWTNHKRYGYKKHSYRLVKKLKLQKMKKNEVYETFSYPESYSPTLPVSRRENNSPSNLPRPSFCMEEYQRAELEEDPILSRTPSPVHPSDFSEHNCQPYYASDGATYGSSSGLCLGNPRADSIHNTYSTDHASAAPPSVTRSPVENDGYIEEGSITKHPSTWSVEAVVLFLKQTDPLALCPLVDLFRSHEIDGKALLLLTSDVLLKHLGVKLGTAVKLCYYIDRLKQGKCFEN

相同性解析による交差性が予測される生物種
Full Name sex comb on midleg-like 1 (Drosophila)
Calculated molecular weight 37 kDa
Observed molecular weight30-35 kDa
GenBank accession numberBC026159
Gene Symbol SCML1
Gene ID (NCBI) 6322
RRIDAB_2153243
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UN30
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Sex comb on midleg-like 1 (SCML1) is a meiosis-specific protein and is an essential component of the meiotic dense body. SCML1 is preferentially expressed in germ stem cells of testis, therefore likely involved in spermatogenesis.

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