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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCML1 Polyclonal antibody (15093-1-AP)

    WB analysis using 15093-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15093-1-AP (SCML1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SCML1 Polyclonal antibody (15093-1-AP)

    IHC staining of mouse testis using 15093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15093-1-AP (SCML1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SCML1 Polyclonal antibody (15093-1-AP)

    IHC staining of mouse testis using 15093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15093-1-AP (SCML1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SCML1 Polyclonal antibody (15093-1-AP)

    IF Staining of A549 using 15093-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SCML1 antibody (15093-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of A549 cells using SCML1 Polyclonal antibody (15093-1-AP)

    FC experiment of A549 using 15093-1-AP

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SCML1 Polyclonal antibody (15093-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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SCML1 Polyclonal antibody

SCML1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 15093-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Sex comb on midleg-like protein 1, SCML 1, Gm47, Gm1792

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15093-1-AP targets SCML1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCML1 fusion protein Ag5107 相同性解析による交差性が予測される生物種
Full Name sex comb on midleg-like 1 (Drosophila)
Calculated molecular weight 37 kDa
Observed molecular weight30-35 kDa
GenBank accession numberBC026159
Gene Symbol SCML1
Gene ID (NCBI) 6322
RRIDAB_2153243
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UN30
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sex comb on midleg-like 1 (SCML1) is a meiosis-specific protein and is an essential component of the meiotic dense body. SCML1 is preferentially expressed in germ stem cells of testis, therefore likely involved in spermatogenesis.

Protocols

Product Specific Protocols
WB protocol for SCML1 antibody 15093-1-APDownload protocol
IHC protocol for SCML1 antibody 15093-1-APDownload protocol
IF protocol for SCML1 antibody 15093-1-APDownload protocol
FC protocol for SCML1 antibody 15093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols