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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SARS2 Polyclonal antibody (17258-1-AP)

    WB analysis using 17258-1-AP (same clone as 17258-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17258-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SARS2 Polyclonal antibody (17258-1-AP)

    WB analysis of HeLa using 17258-1-AP (same clone as 17258-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17258-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using SARS2 Polyclonal antibody (17258-1-AP)

    WB analysis of mouse liver using 17258-1-AP (same clone as 17258-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17258-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SARS2 Polyclonal antibody (17258-1-AP)

    WB analysis of HepG2 using 17258-1-AP (same clone as 17258-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SARS2 Polyclonal antibody (17258-1-AP)

    IHC staining of human lung cancer using 17258-1-AP (same clone as 17258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17258-1-AP (SARS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17258-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SARS2 Polyclonal antibody (17258-1-AP)

    IHC staining of human lung cancer using 17258-1-AP (same clone as 17258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17258-1-AP (SARS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17258-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SARS2 Polyclonal antibody (17258-1-AP)

    IF Staining of HepG2 using 17258-1-AP (same clone as 17258-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17258-1-AP (SARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17258-1-PBS in a different storage buffer formulation.

SARS2 Polyclonal antibody, PBS Only

SARS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17258-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17258-1-PBS targets SARS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10913

Product name: Recombinant human SARS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 169-518 aa of BC042912

Sequence: KLPNQTHPDVPVGDESQARVLHMVGDKPVFSFQPRGHLEIGEKLDIIRQKRLSHVSGHRSYYLRGAGALLQHGLVNFTFNKLLRRGFTPMTVPDLLRGAVFEGCGMTPNANPSQIYNIDPARFKDLNLAGTAEVGLAGYFMDHTVAFRDLPVRMVCSSTCYRAETNTGQEPRGLYRVHHFTKVEMFGVTGPGLEQSSQLLEEFLSLQMEILTELGLHFRVLDMPTQELGLPAYRKFDIEAWMPGRGRFGEVTSASNCTDFQSRRLHIMFQTEAGELQFAHTVNATACAVPRLLIALLESNQQKDGSVLVPPALQSYLGTDRITAPTHVPLQYIGPNQPRKPGLPGQPAVS

相同性解析による交差性が予測される生物種
Full Name seryl-tRNA synthetase 2, mitochondrial
Calculated molecular weight 518 aa, 58 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC042912
Gene Symbol SARS2
Gene ID (NCBI) 54938
RRIDAB_2238863
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP81
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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