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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SARS2 Polyclonal antibody (17258-1-AP)

    WB analysis using 17258-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SARS2 Polyclonal antibody (17258-1-AP)

    WB analysis of HeLa using 17258-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SARS2 Polyclonal antibody (17258-1-AP)

    WB analysis of mouse liver using 17258-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SARS2 Polyclonal antibody (17258-1-AP)

    WB analysis of HepG2 using 17258-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SARS2 Polyclonal antibody (17258-1-AP)

    IHC staining of human lung cancer using 17258-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17258-1-AP (SARS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SARS2 Polyclonal antibody (17258-1-AP)

    IHC staining of human lung cancer using 17258-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17258-1-AP (SARS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SARS2 Polyclonal antibody (17258-1-AP)

    IF Staining of HepG2 using 17258-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17258-1-AP (SARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SARS2 Polyclonal antibody

SARS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17258-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, RIP, ELISA

Synonyms

EC:6.1.1.11, mtSerRS, SARS, SARSM, Serine  tRNA ligase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse liver tissue, HepG2 cells, rat kidney tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17258-1-AP targets SARS2 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10913

Product name: Recombinant human SARS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 169-518 aa of BC042912

Sequence: KLPNQTHPDVPVGDESQARVLHMVGDKPVFSFQPRGHLEIGEKLDIIRQKRLSHVSGHRSYYLRGAGALLQHGLVNFTFNKLLRRGFTPMTVPDLLRGAVFEGCGMTPNANPSQIYNIDPARFKDLNLAGTAEVGLAGYFMDHTVAFRDLPVRMVCSSTCYRAETNTGQEPRGLYRVHHFTKVEMFGVTGPGLEQSSQLLEEFLSLQMEILTELGLHFRVLDMPTQELGLPAYRKFDIEAWMPGRGRFGEVTSASNCTDFQSRRLHIMFQTEAGELQFAHTVNATACAVPRLLIALLESNQQKDGSVLVPPALQSYLGTDRITAPTHVPLQYIGPNQPRKPGLPGQPAVS

相同性解析による交差性が予測される生物種
Full Name seryl-tRNA synthetase 2, mitochondrial
Calculated molecular weight 518 aa, 58 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC042912
Gene Symbol SARS2
Gene ID (NCBI) 54938
RRIDAB_2238863
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP81
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for SARS2 antibody 17258-1-APDownload protocol
IHC protocol for SARS2 antibody 17258-1-APDownload protocol
WB protocol for SARS2 antibody 17258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,RIP

Oncogene

NSUN2 regulates Wnt signaling pathway depending on the m5C RNA modification to promote the progression of hepatocellular carcinoma

Authors - Huiwu Xing
View Article
humanWB

J Biol Chem

Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNAHis mutation.

Authors - Shasha Gong
View Article

EMBO Mol Med

Comprehensive functional testing in fibroblasts has strong utility to diagnose mitochondrial disease.

Authors - Johan L K Van Hove
View Article
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