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Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using S100A9 Monoclonal antibody (68900-1-Ig)

    WB analysis of human saliva using 68900-1-Ig (same clone as 68900-1-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68900-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-468 cells using S100A9 Monoclonal antibody (68900-1-Ig)

    WB analysis of MDA-MB-468 using 68900-1-Ig (same clone as 68900-1-PBS)

    MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68900-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1376 cells using S100A9 Monoclonal antibody (68900-1-Ig)

    WB analysis of HT-1376 using 68900-1-Ig (same clone as 68900-1-PBS)

    HT-1376 cells were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68900-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SK-BR-3 cells using S100A9 Monoclonal antibody (68900-1-Ig)

    WB analysis of SK-BR-3 using 68900-1-Ig (same clone as 68900-1-PBS)

    SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68900-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using S100A9 Monoclonal antibody (68900-1-Ig)

    IF Staining of U2OS using 68900-1-Ig (same clone as 68900-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using S100A9 antibody (68900-1-Ig, Clone: 1E12C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68900-1-PBS in a different storage buffer formulation.

S100A9 Monoclonal antibody, PBS Only

S100A9 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68900-1-PBS
Clone No.1E12C1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

1E12C1, CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68900-1-PBS targets S100A9 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag27025

Product name: Recombinant human S100A9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 25-114 aa of BC047681

Sequence: KLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASHEKMHEGDEGPGHHHKPGLGEGTP

相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A9
Calculated molecular weight 13 kDa
Observed molecular weight14 kDa
GenBank accession numberBC047681
Gene Symbol S100A9
Gene ID (NCBI) 6280
RRIDAB_3670450
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP06702
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer (24 kDa) with an S100A8 partner (S100A8/A9), or as a heterotetramer (28 kDa) with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

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