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Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using S100A9 Monoclonal antibody (68900-1-Ig)

    WB analysis of human saliva using 68900-1-Ig

    human saliva were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-468 cells using S100A9 Monoclonal antibody (68900-1-Ig)

    WB analysis of MDA-MB-468 using 68900-1-Ig

    MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1376 cells using S100A9 Monoclonal antibody (68900-1-Ig)

    WB analysis of HT-1376 using 68900-1-Ig

    HT-1376 cells were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SK-BR-3 cells using S100A9 Monoclonal antibody (68900-1-Ig)

    WB analysis of SK-BR-3 using 68900-1-Ig

    SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using S100A9 Monoclonal antibody (68900-1-Ig)

    IF Staining of U2OS using 68900-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using S100A9 antibody (68900-1-Ig, Clone: 1E12C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

S100A9 Monoclonal antibody

S100A9 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68900-1-Ig
Clone No.1E12C1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

1E12C1, CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman saliva, HT-1376 cells, MDA-MB-468 cells, SK-BR-3 cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68900-1-Ig targets S100A9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag27025

Product name: Recombinant human S100A9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 25-114 aa of BC047681

Sequence: KLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASHEKMHEGDEGPGHHHKPGLGEGTP

相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A9
Calculated molecular weight 13 kDa
Observed molecular weight14 kDa
GenBank accession numberBC047681
Gene Symbol S100A9
Gene ID (NCBI) 6280
RRIDAB_3670450
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP06702
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer (24 kDa) with an S100A8 partner (S100A8/A9), or as a heterotetramer (28 kDa) with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

Protocols

Product Specific Protocols
WB protocol for S100A9 antibody 68900-1-IgDownload protocol
IF protocol for S100A9 antibody 68900-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols