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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®555-conjugated S100A6 Polyclonal antibody (CL555-10245)

    IF Staining of MCF-7 using CL555-10245

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CoraLite®555 S100A6 antibody (CL555-10245) at dilution of 1:20.

  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated S100A6 Polyclonal antibody

S100A6 Polyclonal Antibody for IF/ICC
Cat No. CL555-10245

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

2A9, 5B10, CABP, CACY, Calcyclin, MLN 4, PRA, Protein S100 A6, S100A6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-10245 targets S100A6 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0385

Product name: Recombinant human S100A6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-90 aa of BC001431

Sequence: MACPLDQAIGLLVAIFHKYSGREGDKHTLSKKELKELIQKELTIGSKLQDAEIARLMEDLDRNKDQEVNFQEYVTFLGALALIYNEALKG

相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A6
Calculated molecular weight 10 kDa
GenBank accession numberBC001431
Gene Symbol S100A6
Gene ID (NCBI) 6277
RRIDAB_2919584
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP06703
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. The antibody is specific for S100A6.

Protocols

Product Specific Protocols
IF protocol for CL555 S100A6 antibody CL555-10245Download protocol
Standard Protocols
Click here to view our Standard Protocols