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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using S100A14 Recombinant antibody (83559-5-RR)

    WB analysis of MCF-7 using 83559-5-RR

    WB result of S100A14 antibody (83559-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A14 transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using S100A14 Recombinant antibody (83559-5-RR)

    WB analysis using 83559-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83559-5-RR (S100A14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using S100A14 Recombinant antibody (83559-5-RR)

    IHC staining of human ovary cancer using 83559-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using S100A14 Recombinant antibody (83559-5-RR)

    IHC staining of human ovary cancer using 83559-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using S100A14 Recombinant antibody (83559-5-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83559-5-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using S100A14 Recombinant antibody (83559-5-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83559-5-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using S100A14 Recombinant antibody (83559-5-RR)

    FC experiment of HeLa using 83559-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug S100A14 Recombinant antibody (83559-5-RR, Clone:240528B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of MCF-7 cells using S100A14 Recombinant antibody (83559-5-RR)

    FC experiment of MCF-7 using 83559-5-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug S100A14 Recombinant antibody (83559-5-RR, Clone:240528B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83559-5-RR

    Affinity and Kinetic Characterization of 83559-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83559-5-RR against Human S100A14 were performed. The affinity constant is 4.90 nM.

  • Featured Product
  • KD/KO Validated

S100A14 Recombinant monoclonal antibody

S100A14 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83559-5-RR
Clone No.240528B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

240528B6, Protein S100 A14, Protein S100-A14, S100 calcium-binding protein A14, S100A15

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse stomach tissue, MCF-7 cells, rat stomach tissues, human saliva samples
Positive IHC detected inhuman ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83559-5-RR targets S100A14 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0757

Product name: Recombinant human S100A14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-104 aa of BC005019

Sequence: MGQCRSANAEDAQEFSDVERAIETLIKNFHQYSVEGGKETLTPSELRDLVTQQLPHLMPSNCGLEEKIANLGSCNDSKLEFRSFWELIGEAAKSVKLERPVRGH

相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A14
Calculated molecular weight 12 kDa
Observed molecular weight12 kDa
GenBank accession numberBC005019
Gene Symbol S100A14
Gene ID (NCBI) 57402
RRIDAB_3671175
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9HCY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).7

Protocols

Product Specific Protocols
FC protocol for S100A14 antibody 83559-5-RRDownload protocol
IHC protocol for S100A14 antibody 83559-5-RRDownload protocol
WB protocol for S100A14 antibody 83559-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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