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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using S100A14 Recombinant monoclonal antibody (83559-5-RR)

    WB analysis of MCF-7 using 83559-5-RR (same clone as 83559-5-PBS)

    WB result of S100A14 antibody (83559-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A14 transfected MCF-7 cells. This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using S100A14 Recombinant monoclonal antibody (83559-5-RR)

    WB analysis using 83559-5-RR (same clone as 83559-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83559-5-RR (S100A14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using S100A14 Recombinant monoclonal antibody (83559-5-RR)

    IHC staining of human ovary cancer using 83559-5-RR (same clone as 83559-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using S100A14 Recombinant monoclonal antibody (83559-5-RR)

    IHC staining of human ovary cancer using 83559-5-RR (same clone as 83559-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using S100A14 Recombinant monoclonal antibody (83559-5-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83559-5-RR (same clone as 83559-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using S100A14 Recombinant monoclonal antibody (83559-5-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83559-5-RR (same clone as 83559-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using S100A14 Recombinant monoclonal antibody (83559-5-RR)

    FC experiment of HeLa using 83559-5-RR (same clone as 83559-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug S100A14 Recombinant antibody (83559-5-RR, Clone:240528B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using S100A14 Recombinant monoclonal antibody (83559-5-RR)

    FC experiment of MCF-7 using 83559-5-RR (same clone as 83559-5-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug S100A14 Recombinant antibody (83559-5-RR, Clone:240528B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83559-5-RR

    Affinity and Kinetic Characterization of 83559-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83559-5-RR against Human S100A14 were performed. The affinity constant is 4.90 nM.

  • Featured Product
  • KD/KO Validated

S100A14 Recombinant monoclonal antibody, PBS Only

S100A14 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83559-5-PBS
Clone No.240528B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

240528B6, Protein S100 A14, Protein S100-A14, S100 calcium-binding protein A14, S100A15

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83559-5-PBS targets S100A14 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0757

Product name: Recombinant human S100A14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-104 aa of BC005019

Sequence: MGQCRSANAEDAQEFSDVERAIETLIKNFHQYSVEGGKETLTPSELRDLVTQQLPHLMPSNCGLEEKIANLGSCNDSKLEFRSFWELIGEAAKSVKLERPVRGH

相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A14
Calculated molecular weight 12 kDa
Observed molecular weight12 kDa
GenBank accession numberBC005019
Gene Symbol S100A14
Gene ID (NCBI) 57402
RRIDAB_3671175
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9HCY8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).7

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