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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using S100A1 Polyclonal antibody (16027-1-AP)

    WB analysis of human heart using 16027-1-AP (same clone as 16027-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 16027-1-AP (S100A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using S100A1 Polyclonal antibody (16027-1-AP)

    WB analysis of human heart using 16027-1-AP (same clone as 16027-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 16027-1-AP (S100A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human malignant melanoma using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 10x lens).. This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human malignant melanoma using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 40x lens).. This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human tonsillitis using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human tonsillitis using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of rat brain using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of rat brain using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human thyroid cancer using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human thyroid cancer using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human kidney using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16027-1-AP (S100A1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human kidney using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16027-1-AP (S100A1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human appendicitis using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human appendicitis using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human appendicitis using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human appendicitis using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human tonsillitis using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human tonsillitis using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human ovary tumor using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human ovary tumor using 16027-1-AP (same clone as 16027-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human ovary cancer tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IF Staining of human ovary cancer using 16027-1-AP (same clone as 16027-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary cancer cells using 16027-1-AP (S100A1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human ovary cancer tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IF Staining of human ovary cancer using 16027-1-AP (same clone as 16027-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary cancer cells using 16027-1-AP (S100A1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16027-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

S100A1 Polyclonal antibody, PBS Only

S100A1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 16027-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Protein S100 A1, Protein S100-A1, S 100 protein alpha chain, S 100 protein subunit alpha, S100 calcium-binding protein A1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16027-1-PBS targets S100A1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8871

Product name: Recombinant human S100A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-94 aa of BC014392

Sequence: MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDVDAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS

相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A1
Calculated molecular weight 94 aa, 11 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC014392
Gene Symbol S100A1
Gene ID (NCBI) 6271
RRIDAB_2183214
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP23297
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

S100A1 belongs to the S100 protein family, which represents the largest group among EF-hand Ca2+-binding proteins exclusively expressed in vertebrates. S100 proteins have been described as central regulators of numerous cellular and molecular processes, often operating in a cell-type-specific manner (PMID: 11991838). S100A1 is a central regulator of Ca2+-driven networks that orchestrates compartmentalized Ca2+ fluxes in the heart (PMID: 22336719, PMID: 26021637). S100A1 is primarily expressed in cardiomyocytes (CM). Endothelial cells (EC) express significantly lower S100A1 levels, whereas fibroblasts do not express S100A1 (PMID: 38641766).

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