Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis of human heart using 16027-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 16027-1-AP (S100A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 16027-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 16027-1-AP (S100A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human malignant melanoma using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 10x lens)..
IHC staining of human malignant melanoma using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 40x lens)..
IHC staining of human tonsillitis using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16027-1-AP (S100A1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16027-1-AP (S100A1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human appendicitis using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 16027-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human ovary cancer using 16027-1-AP
Immunofluorescent analysis of (4% PFA) fixed human ovary cancer cells using 16027-1-AP (S100A1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of human ovary cancer using 16027-1-AP
Immunofluorescent analysis of (4% PFA) fixed human ovary cancer cells using 16027-1-AP (S100A1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

S100A1 Polyclonal antibody

S100A1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 16027-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Protein S100 A1, S 100 protein alpha chain, S 100 protein subunit alpha, S100, S100 alpha, S100A, S100A1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human heart tissue
Positive IHC detected in	human malignant melanoma tissue, human kidney tissue, human ovary tumor tissue, human appendicitis tissue, human tonsillitis tissue, rat brain tissue, human thyroid cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human ovary cancer tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below
IF	See 6 publications below

Product Information

16027-1-AP targets S100A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8871 Product name: Recombinant human S100A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-94 aa of BC014392 Sequence: MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDVDAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS 相同性解析による交差性が予測される生物種
Full Name	S100 calcium binding protein A1
Calculated molecular weight	94 aa, 11 kDa
Observed molecular weight	10 kDa
GenBank accession number	BC014392
Gene Symbol	S100A1
Gene ID (NCBI)	6271
RRID	AB_2183214
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P23297
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for S100A1 antibody 16027-1-AP	Download protocol
IHC protocol for S100A1 antibody 16027-1-AP	Download protocol
WB protocol for S100A1 antibody 16027-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	IF	Biomaterials Biocompatibility and therapeutic efficacy of crosslinked hydrogel filled 3D-printed nerve conduit for sacral nerve injury repair Authors - Jiajia Lu View Article
rat	IF	Biomaterials Oriented nanofibrous P(MMD-co-LA)/Deferoxamine nerve scaffold facilitates peripheral nerve regeneration by regulating macrophage phenotype and revascularization. Authors - Xianzhen Dong View Article
human	IHC	Mol Neurobiol Anti-Apoptotic Effect of IGF1 on Schwann Exposed to Hyperglycemia is Mediated by Neuritin, a Novel Neurotrophic Factor. Authors - Lingfei Yan View Article
rat	WB	Front Pharmacol Reynoutrin Improves Ischemic Heart Failure in Rats Via Targeting S100A1. Authors - Wenkai Yang KD Validated View Article
human	WB	Circ Heart Fail S100A1 in Human Heart Failure: Lack of Recovery Following LVAD Support. Authors - Mosi K Bennett View Article
	IF	Adv Mater Photo-Responsive H2S Composite System Regulates the Nerve Regeneration Microenvironment Through Multiple Pathways Authors - Yuanfang Huo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human heart using 16027-1-AP

WB analysis of human heart using 16027-1-AP

IHC staining of human malignant melanoma using 16027-1-AP

IHC staining of human malignant melanoma using 16027-1-AP

IHC staining of human tonsillitis using 16027-1-AP

IHC staining of rat brain using 16027-1-AP

IHC staining of rat brain using 16027-1-AP

IHC staining of human thyroid cancer using 16027-1-AP

IHC staining of human thyroid cancer using 16027-1-AP

IHC staining of human tonsillitis using 16027-1-AP

IHC staining of human kidney using 16027-1-AP

IHC staining of human kidney using 16027-1-AP

IHC staining of human appendicitis using 16027-1-AP

IHC staining of human appendicitis using 16027-1-AP

IHC staining of human appendicitis using 16027-1-AP

IHC staining of human appendicitis using 16027-1-AP

IHC staining of human tonsillitis using 16027-1-AP

IHC staining of human tonsillitis using 16027-1-AP

IHC staining of human ovary tumor using 16027-1-AP

IHC staining of human ovary tumor using 16027-1-AP

IF Staining of human ovary cancer using 16027-1-AP

IF Staining of human ovary cancer using 16027-1-AP

S100A1 Polyclonal antibody

S100A1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Biocompatibility and therapeutic efficacy of crosslinked hydrogel filled 3D-printed nerve conduit for sacral nerve injury repair

Oriented nanofibrous P(MMD-co-LA)/Deferoxamine nerve scaffold facilitates peripheral nerve regeneration by regulating macrophage phenotype and revascularization.

Anti-Apoptotic Effect of IGF1 on Schwann Exposed to Hyperglycemia is Mediated by Neuritin, a Novel Neurotrophic Factor.

Reynoutrin Improves Ischemic Heart Failure in Rats Via Targeting S100A1.

S100A1 in Human Heart Failure: Lack of Recovery Following LVAD Support.

Photo-Responsive H2S Composite System Regulates the Nerve Regeneration Microenvironment Through Multiple Pathways