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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S100 Beta Monoclonal antibody, PBS Only

S100 Beta Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G10F4

Cat no : 66616-1-PBS

Synonyms

NEF, Protein S100 B, S 100 protein beta chain, S 100 protein subunit beta, S100, S100 Beta, S100 calcium binding protein B, S100B, S100beta

Validation Data Gallery

Applications:
  • WB analysis of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

    WB analysis of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66616-1-Ig (S100B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • WB analysis of C6 using 66616-1-Ig (same clone as 66616-1-PBS)

    WB analysis of C6 using 66616-1-Ig (same clone as 66616-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 66616-1-Ig (S100B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • WB analysis of pig brain using 66616-1-Ig (same clone as 66616-1-PBS)

    WB analysis of pig brain using 66616-1-Ig (same clone as 66616-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66616-1-Ig (S100B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 66616-1-Ig (same clone as 66616-1-PBS)

    IHC staining of human malignant melanoma using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100B antibody included
  • IHC staining of human malignant melanoma using 66616-1-Ig (same clone as 66616-1-PBS)

    IHC staining of human malignant melanoma using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100B antibody included
  • IHC staining of mouse brain using 66616-1-Ig (same clone as 66616-1-PBS)

    IHC staining of mouse brain using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100B antibody included
  • IHC staining of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

    IHC staining of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100B antibody included
  • IHC staining of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

    IHC staining of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100B antibody included
  • IHC staining of mouse brain using 66616-1-Ig (same clone as 66616-1-PBS)

    IHC staining of mouse brain using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100B antibody included
  • IHC staining of human appendicitis using 66616-1-Ig (same clone as 66616-1-PBS)

    IHC staining of human appendicitis using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66616-1-Ig (S100 beta antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100B antibody included
  • IHC staining of human appendicitis using 66616-1-Ig (same clone as 66616-1-PBS)

    IHC staining of human appendicitis using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66616-1-Ig (S100 beta antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100B antibody included
  • IHC staining of mouse brain using 66616-1-Ig (same clone as 66616-1-PBS)

    IHC staining of mouse brain using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100B antibody included
  • IHC staining of mouse brain using 66616-1-Ig (same clone as 66616-1-PBS)

    IHC staining of mouse brain using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66616-1-Ig (S100B antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100B antibody included
  • IF Staining of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

    IF Staining of rat brain using 66616-1-Ig (same clone as 66616-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using S100 Beta antibody (66616-1-Ig, Clone: 1G10F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66616-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy S100B Ready-To-Use IHC Kit. S100B primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66616-1-PBS targets S100 Beta in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Reactivity Human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen S100 Beta fusion protein Ag7440 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein B
Calculated molecular weight 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC001766
Gene symbol S100B
Gene ID (NCBI) 6285
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new biomarker for mood disorders.