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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated S1 tag Monoclonal an (CL488-66165)

    WB analysis using CL488-66165

    S1 tagged protein lysate was subjected to SDS PAGE followed by western blot with CL488-66165 (S1 tag antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite® Plus 488-conjugated S1 tag Monoclonal antibody

S1 tag Monoclonal Antibody for WB
Cat No. CL488-66165
Clone No.1A9B5

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

WB

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66165 targets S1 tag in WB applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name S1 Tag
Gene Symbol S1 Tag
Gene ID (NCBI) 7
RRIDAB_2935605
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S1 tag, with sequence NANNPDWDF, is derived from the hepatitis B virus preS1 region. S1 tag is widely used in various protein expression systems. This antibody can detect the over-expressed fusion proteins containing S1 epitope tag.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 S1 tag antibody CL488-66165Download protocol
Standard Protocols
Click here to view our Standard Protocols