Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-85839-4 targets RUFY1 in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag4324 Product name: Recombinant human RUFY1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 219-600 aa of BC032571 Sequence: TKIDGLEKTNSKLQEELSAATDRICSLQEEQQQLREQNELIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLELEKELELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKAQNAESSLQQKNEAITSFEGKTNQVMSSMKQMEERLQHSERARQGAEERSHKLQQELGGRIGALQLQLSQLHEQCSSLEKELKSEKEQRQALQRELQHEKDTSSLLRMELQQVEGLKKELRELQDEKAELQKICEEQEQALQEMGLHLSQSKLKMEDIKEVNQALKGHAWLKDDEATHCRQCEKEFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCHTLLLQRCSSTAS 相同性解析による交差性が予測される生物種 |
| Full Name | RUN and FYVE domain containing 1 |
| Calculated molecular weight | 708 aa, 80 kDa |
| Observed molecular weight | 80 kDa, 69 kDa |
| GenBank accession number | BC032571 |
| Gene Symbol | RUFY1 |
| Gene ID (NCBI) | 80230 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96T51 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RUFY1 antibody CL488-85839-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |