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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RTN4IP1 Polyclonal antibody (15636-1-AP)

    WB analysis using 15636-1-AP (same clone as 15636-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15636-1-AP (RTN4IP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15636-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RTN4IP1 Polyclonal antibody (15636-1-AP)

    IF Staining of HepG2 using 15636-1-AP (same clone as 15636-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RTN4IP1 antibody (15636-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15636-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RTN4IP1 Polyclonal antibody (15636-1-AP)

    IF Staining of HepG2 using 15636-1-AP (same clone as 15636-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RTN4IP1 antibody (15636-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 15636-1-PBS in a different storage buffer formulation.

RTN4IP1 Polyclonal antibody, PBS Only

RTN4IP1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 15636-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

NIMP, NOGO-interacting mitochondrial protein, Reticulon-4-interacting protein 1, mitochondrial

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15636-1-PBS targets RTN4IP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8100

Product name: Recombinant human RTN4IP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-396 aa of BC006399

Sequence: MEFLKTCVLRRNACTAVCFWRSKVVQKPSVRRISTTSPRSTVMPAWVIDKYGKNEVLRFTQNMMMPIIHYPNEVIVKVHAASVNPIDVNMRSGYGATALNMKRDPLHVKIKGEEFPLTLGRDVSGVVMECGLDVKYFKPGDEVWAAVPPWKQGTLSEFVVVSGNEVSHKPKSLTHTQAASLPYVALTAWSAINKVGGLNDKNCTGKRVLILGASGGVGTFAIQVMKAWDAHVTAVCSQDASELVRKLGADDVIDYKSGSVEEQLKSLKPFDFILDNVGGSTETWAPDFLKKWSGATYVTLVTPFLLNMDRLGIADGMLQTGVTVGSKALKHFWKGVHYRWAFFMASGPCLDDIAELVDAGKIRPVIEQTFPFSKVPEAFLKVERGHARGKTVINVV

相同性解析による交差性が予測される生物種
Full Name reticulon 4 interacting protein 1
Calculated molecular weight 396 aa, 44 kDa
Observed molecular weight32-44 kDa, 24 kDa
GenBank accession numberBC006399
Gene Symbol RTN4IP1
Gene ID (NCBI) 84816
RRIDAB_3669217
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWV3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RTN4IP1 (Reticulon-4-interacting protein 1, mitochondrial), also known as NIMP. It colocalizes with the endoplasmic reticulum HSPA5 at spots corresponding to contacts with mitochondria (PMID: 26593267), which is widely expressed in mitochondria-enriched tissues. Found in heart, muscle, kidney, liver, brain and placenta (PMID: 12067236). The protein plays a role in the regulation of retinal ganglion cell (RGC) neurite outgrowth, and hence in the development of the inner retina and optic nerve. Appears to be a potent inhibitor of regeneration following spinal cord injury. The protein has three isoforms with molecular weights of 43 kDa, 32 kDa and 24 kDa respectively.

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