Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, Jurkat cells, MCF-7 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15636-1-AP targets RTN4IP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8100 Product name: Recombinant human RTN4IP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-396 aa of BC006399 Sequence: MEFLKTCVLRRNACTAVCFWRSKVVQKPSVRRISTTSPRSTVMPAWVIDKYGKNEVLRFTQNMMMPIIHYPNEVIVKVHAASVNPIDVNMRSGYGATALNMKRDPLHVKIKGEEFPLTLGRDVSGVVMECGLDVKYFKPGDEVWAAVPPWKQGTLSEFVVVSGNEVSHKPKSLTHTQAASLPYVALTAWSAINKVGGLNDKNCTGKRVLILGASGGVGTFAIQVMKAWDAHVTAVCSQDASELVRKLGADDVIDYKSGSVEEQLKSLKPFDFILDNVGGSTETWAPDFLKKWSGATYVTLVTPFLLNMDRLGIADGMLQTGVTVGSKALKHFWKGVHYRWAFFMASGPCLDDIAELVDAGKIRPVIEQTFPFSKVPEAFLKVERGHARGKTVINVV 相同性解析による交差性が予測される生物種 |
| Full Name | reticulon 4 interacting protein 1 |
| Calculated molecular weight | 396 aa, 44 kDa |
| Observed molecular weight | 32-44 kDa, 24 kDa |
| GenBank accession number | BC006399 |
| Gene Symbol | RTN4IP1 |
| Gene ID (NCBI) | 84816 |
| RRID | AB_3669217 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WWV3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RTN4IP1 (Reticulon-4-interacting protein 1, mitochondrial), also known as NIMP. It colocalizes with the endoplasmic reticulum HSPA5 at spots corresponding to contacts with mitochondria (PMID: 26593267), which is widely expressed in mitochondria-enriched tissues. Found in heart, muscle, kidney, liver, brain and placenta (PMID: 12067236). The protein plays a role in the regulation of retinal ganglion cell (RGC) neurite outgrowth, and hence in the development of the inner retina and optic nerve. Appears to be a potent inhibitor of regeneration following spinal cord injury. The protein has three isoforms with molecular weights of 43 kDa, 32 kDa and 24 kDa respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RTN4IP1 antibody 15636-1-AP | Download protocol |
| WB protocol for RTN4IP1 antibody 15636-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
