Validation Data Gallery
Tested Applications
Positive WB detected in | mouse heart tissue, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
86162-1-RR targets RTN2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | RTN2 fusion protein Ag1655 相同性解析による交差性が予測される生物種 |
Full Name | reticulon 2 |
Calculated molecular weight | 59 kDa, 51 kDa, 22 kDa |
Observed molecular weight | 20-23 kDa |
GenBank accession number | BC014244 |
Gene Symbol | RTN2 |
Gene ID (NCBI) | 6253 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O75298 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. Human RTN2 gene produces three isoforms termed RTN2-A, RTN2-B, and RTN2-C. Isoform RTN2-C has a calculated molecular weight of 22 kDa and is highly expressed in skeletal muscle.
Protocols
Product Specific Protocols | |
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WB protocol for RTN2 antibody 86162-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |