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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RTN2 Recombinant antibody (86162-1-RR)

    WB analysis using 86162-1-RR (same clone as 86162-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86162-1-RR (RTN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86162-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RTN2 Recombinant antibody (86162-1-RR)

    WB analysis using 86162-1-RR (same clone as 86162-1-PBS)

    Various cell lysates were subjected to SDS PAGE followed by western blot with 86162-1-RR (RTN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86162-1-PBS in a different storage buffer formulation.

  • Sample test of MP02385-1

    Sample test of MP02385-1

    The mean RTN2 concentration was determined to be 4623.0 ng/mL in Mouse skeletal muscle tissue extract based on a 4.1 mg/mL extract load.

  • Cytometric bead array standard curve of MP02385-1

    Cytometric bead array standard curve of MP02385-1

    Cytometric bead array standard curve of MP02385-1, RTN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86162-1-PBS. Detection antibody: 86162-2-PBS. Standard: Ag1655. Range: 0.469-60 ng/mL.

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RTN2 Recombinant antibody, PBS Only (Capture)

RTN2 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 86162-1-PBS
Clone No.250823C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Neuroendocrine-specific protein-like 1, Neuroendocrine-specific protein-like I, NSPL1, NSPLI, NSP-like protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86162-1-PBS targets RTN2 as part of a matched antibody pair:

MP02385-1: 86162-1-PBS capture and 86162-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RTN2 fusion protein Ag1655 相同性解析による交差性が予測される生物種
Full Name reticulon 2
Calculated molecular weight 59 kDa, 51 kDa, 22 kDa
Observed molecular weight 20-23 kDa
GenBank accession numberBC014244
Gene Symbol RTN2
Gene ID (NCBI) 6253
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75298
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. Human RTN2 gene produces three isoforms termed RTN2-A, RTN2-B, and RTN2-C. Isoform RTN2-C has a calculated molecular weight of 22 kDa and is highly expressed in skeletal muscle.