Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of NIH/3T3 using 12530-1-AP (same clone as 12530-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12530-1-AP (RRAS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.
WB analysis of mouse pancreas using 12530-1-AP (same clone as 12530-1-PBS)
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12530-1-AP (RRAS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.
IP experiment of NIH/3T3 using 12530-1-AP (same clone as 12530-1-PBS)
IP result of anti-RRAS2 (IP:12530-1-AP, 4ug; Detection:12530-1-AP 1:800) with NIH/3T3 cells lysate 4000ug. This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 12530-1-AP (same clone as 12530-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 12530-1-AP (same clone as 12530-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 12530-1-AP (same clone as 12530-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 12530-1-AP (same clone as 12530-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12530-1-AP (RRAS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 12530-1-AP (same clone as 12530-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12530-1-AP (RRAS2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 12530-1-AP (same clone as 12530-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12530-1-AP (RRAS2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 12530-1-AP (same clone as 12530-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 12530-1-AP (RRAS2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 12530-1-AP (same clone as 12530-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RRAS2 antibody (12530-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.
FC experiment of A431 using 12530-1-AP (same clone as 12530-1-PBS)
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RRAS2 (12530-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.
×
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RRAS2 (12530-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 12530-1-PBS in a different storage buffer formulation.

RRAS2 Polyclonal antibody, PBS Only

RRAS2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 12530-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

EC:3.6.5.-, Ras like protein TC21, Ras related protein R Ras2, Ras-like protein TC21, Ras-related protein R-Ras2

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12530-1-PBS targets RRAS2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3226 Product name: Recombinant human RRAS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-204 aa of BC013106 Sequence: MAAAGWRDGSGQEKYRLVVVGGGGVGKSALTIQFIQSYFVTDYDPTIEDSYTKQCVIDDRAARLDILDTAGQEEFGAMREQYMRTGEGFLLVFSVTDRGSFEEIYKFQRQILRVKDRDEFPMILIGNKADLDHQRQVTQEEGQQLARQLKVTYMEASAKIRMNVDQAFHELVRVIRKFQEQECPPSPEPTRKEKDKKGCHCVIF 相同性解析による交差性が予測される生物種
Full Name	related RAS viral (r-ras) oncogene homolog 2
Calculated molecular weight	204 aa, 23 kDa
Observed molecular weight	23 kDa
GenBank accession number	BC013106
Gene Symbol	RRAS2
Gene ID (NCBI)	22800
RRID	AB_2180206
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62070
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Ras-related protein R-Ras2 (RRAS2) is also named TC21 and Teratocarcinoma oncogene. The RRAS2 member of the RAS-related subfamily, also known as TC21, is a close relative of the classic RAS GTPases (PMID: 3898776). RRAS2, a member of the R-Ras subfamily of Ras-like low-molecular-weight GTPases, is located in the plasma membrane and functions as a signal transducer (PMID: 38601074). RRAS2 is GTP-binding protein with GTPase activity, involved in the regulation of MAPK signaling pathway and thereby controlling multiple cellular processes (PMID: 31130282).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of NIH/3T3 using 12530-1-AP (same clone as 12530-1-PBS)

WB analysis of mouse pancreas using 12530-1-AP (same clone as 12530-1-PBS)

IP experiment of NIH/3T3 using 12530-1-AP (same clone as 12530-1-PBS)

IHC staining of mouse heart using 12530-1-AP (same clone as 12530-1-PBS)

IHC staining of mouse heart using 12530-1-AP (same clone as 12530-1-PBS)

IHC staining of human lung cancer using 12530-1-AP (same clone as 12530-1-PBS)

IHC staining of human lung cancer using 12530-1-AP (same clone as 12530-1-PBS)

IHC staining of human heart using 12530-1-AP (same clone as 12530-1-PBS)

IHC staining of human heart using 12530-1-AP (same clone as 12530-1-PBS)

IHC staining of human lung cancer using 12530-1-AP (same clone as 12530-1-PBS)

IF Staining of A431 using 12530-1-AP (same clone as 12530-1-PBS)

FC experiment of A431 using 12530-1-AP (same clone as 12530-1-PBS)

RRAS2 Polyclonal antibody, PBS Only

RRAS2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

Able^™