Various lysates were subjected to SDS PAGE followed by western blot with 87667-1-RR (RRAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87667-1-PBS in a different storage buffer formulation.

The mean RRAS concentration was determined to be 1,971.10 ng/mL in Jurkat cell extract based on a 1.20 mg/mL extract load, 926.11 ng/mL in U2OS cell extract based on a 1.20 mg/mL extract load, 1,001.10 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load, 1,377.33 ng/mL in BxPC-3 cell extract based on a 1.20 mg/mL extract load, 707.70 ng/mL in Raji cell extract based on a 1.20 mg/mL extract load and 670.37 ng/mL in HL-60 cell extract based on a 1.50 mg/mL extract load.

Sandwich ELISA standard curve of MP03286-1, Human RRAS Recombinant Matched Antibody Pair - PBS only. 87667-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg6612. 87667-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.