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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS4X Recombinant antibody (85040-5-RR)

    WB analysis using 85040-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85040-5-RR (SCAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RPS4X Recombinant antibody (85040-5-RR)

    IF Staining of MCF-7 using 85040-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SCAR antibody (85040-5-RR, Clone: 242697E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using RPS4X Recombinant antibody (85040-5-RR)

    FC experiment of HeLa using 85040-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPS4X Recombinant antibody (85040-5-RR, Clone:242697E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 85040-5-RR

    Affinity and Kinetic Characterization of 85040-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85040-5-RR against Human RPS4X were performed. The affinity constant is below 1 pM.

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RPS4X Recombinant antibody

RPS4X Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 85040-5-RR
Clone No.242697E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

242697E12, 40S ribosomal protein S4, Small ribosomal subunit protein eS4, X isoform

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-29 cells, HepG2 cells, HeLa cells, MCF-7 cells, mouse spleen tissue, mouse brain tissue, rat liver tissue
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85040-5-RR targets RPS4X in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPS4X fusion protein Ag6513 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S4, X-linked
Calculated molecular weight 263 aa, 30 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC000472
Gene Symbol RPS4X
Gene ID (NCBI) 6191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62701
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). (PMID:8358435)

Protocols

Product Specific Protocols
WB protocol for RPS4X antibody 85040-5-RRDownload protocol
IF protocol for RPS4X antibody 85040-5-RRDownload protocol
FC protocol for RPS4X antibody 85040-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols