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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS4X Recombinant antibody (85040-5-RR)

    WB analysis using 85040-5-RR (same clone as 85040-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85040-5-RR (SCAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85040-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RPS4X Recombinant antibody (85040-5-RR)

    IF Staining of MCF-7 using 85040-5-RR (same clone as 85040-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SCAR antibody (85040-5-RR, Clone: 242697E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85040-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using RPS4X Recombinant antibody (85040-5-RR)

    FC experiment of HeLa using 85040-5-RR (same clone as 85040-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPS4X Recombinant antibody (85040-5-RR, Clone:242697E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85040-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85040-5-RR

    Affinity and Kinetic Characterization of 85040-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85040-5-RR against Human RPS4X were performed. The affinity constant is below 1 pM.

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RPS4X Recombinant antibody, PBS Only

RPS4X Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 85040-5-PBS
Clone No.242697E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

242697E12, 40S ribosomal protein S4, Small ribosomal subunit protein eS4, X isoform

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85040-5-PBS targets RPS4X in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPS4X fusion protein Ag6513 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S4, X-linked
Calculated molecular weight 263 aa, 30 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC000472
Gene Symbol RPS4X
Gene ID (NCBI) 6191
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62701
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). (PMID:8358435)