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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS3 Recombinant antibody (84947-4-RR)

    WB analysis using 84947-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84947-4-RR (RPS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPS3 Recombinant antibody (84947-4-RR)

    IHC staining of human colon cancer using 84947-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84947-4-RR (RPS3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RPS3 Recombinant antibody (84947-4-RR)

    IF Staining of NIH/3T3 using 84947-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RPS3 antibody (84947-4-RR, Clone: 242504F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84947-4-RR

    Affinity and Kinetic Characterization of 84947-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84947-4-RR against Human RPS3 were performed. The affinity constant is below 1 pM.

RPS3 Recombinant monoclonal antibody

RPS3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84947-4-RR
Clone No.242504F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

242504F3, 40S ribosomal protein S3, EC:4.2.99.18, FLJ26283, FLJ27450

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, COLO 320 cells, NIH/3T3 cells, mouse brain tissue, human brain tissue,
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84947-4-RR targets RPS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7346

Product name: Recombinant human RPS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-243 aa of BC003137

Sequence: MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSIVEPKDEILPTTPISEQKGGKPEPPAMPQPVPTA

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S3
Calculated molecular weight 27 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC003137
Gene Symbol RPS3
Gene ID (NCBI) 6188
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP23396
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.

Protocols

Product Specific Protocols
IF protocol for RPS3 antibody 84947-4-RRDownload protocol
IHC protocol for RPS3 antibody 84947-4-RRDownload protocol
WB protocol for RPS3 antibody 84947-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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