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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS3 Recombinant antibody (84947-4-RR)

    WB analysis using 84947-4-RR (same clone as 84947-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84947-4-RR (RPS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84947-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RPS3 Recombinant antibody (84947-4-RR)

    IHC staining of human colon cancer using 84947-4-RR (same clone as 84947-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84947-4-RR (RPS3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84947-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RPS3 Recombinant antibody (84947-4-RR)

    IF Staining of NIH/3T3 using 84947-4-RR (same clone as 84947-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RPS3 antibody (84947-4-RR, Clone: 242504F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84947-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84947-4-RR

    Affinity and Kinetic Characterization of 84947-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84947-4-RR against Human RPS3 were performed. The affinity constant is below 1 pM.

RPS3 Recombinant monoclonal antibody, PBS Only

RPS3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84947-4-PBS
Clone No.242504F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242504F3, 40S ribosomal protein S3, EC:4.2.99.18, FLJ26283, FLJ27450

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84947-4-PBS targets RPS3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7346

Product name: Recombinant human RPS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-243 aa of BC003137

Sequence: MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSIVEPKDEILPTTPISEQKGGKPEPPAMPQPVPTA

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S3
Calculated molecular weight 27 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC003137
Gene Symbol RPS3
Gene ID (NCBI) 6188
RRIDAB_3743873
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP23396
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival.

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