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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS17 Recombinant antibody (83848-4-RR)

    WB analysis using 83848-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83848-4-RR (RPS17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RPS17 Recombinant monoclonal antibody (83848-4-RR)

    IF Staining of U2OS using 83848-4-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RPS17 antibody (83848-4-RR, Clone: 240851H3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using RPS17 Recombinant monoclonal antibody (83848-4-RR)

    FC experiment of HeLa using 83848-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPS17 Recombinant antibody (83848-4-RR, Clone:240851H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83848-4-RR

    Affinity and Kinetic Characterization of 83848-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83848-4-RR against Human RPS17 were performed. The affinity constant is 21.2 pM.

RPS17 Recombinant monoclonal antibody

RPS17 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83848-4-RR
Clone No.240851H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240851H3, 40S ribosomal protein S17, DBA4, ribosomal protein S17, RPS17L

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, HEK-293 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83848-4-RR targets RPS17 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9334

Product name: Recombinant human RPS17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-135 aa of BC009407

Sequence: MGRVRTKTVKKAARVIIEKYYTRLGNDFHTNKRVCEEIAIIPSKKLRNKIAGYVTHLMKRIQRGPVRGISIKLQEEERERRDNYVPEVSALDQEIIEVDPDTKEMLKLLDFGSLSNLQVTQPTVGMNFKTPRGPV

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S17
Calculated molecular weight 135 aa, 16 kDa
Observed molecular weight16 kDa
GenBank accession numberBC009407
Gene Symbol RPS17
Gene ID (NCBI) 6218
RRIDAB_3671434
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP08708
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS17 is a component of the small ribosomal subunit, the ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

Protocols

Product Specific Protocols
FC protocol for RPS17 antibody 83848-4-RRDownload protocol
IF protocol for RPS17 antibody 83848-4-RRDownload protocol
WB protocol for RPS17 antibody 83848-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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