Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, Jurkat cells, HEK-293 cells |
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83848-4-RR targets RPS17 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | RPS17 fusion protein Ag9334 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S17 |
| Calculated molecular weight | 135 aa, 16 kDa |
| Observed molecular weight | 16 kDa |
| GenBank accession number | BC009407 |
| Gene Symbol | RPS17 |
| Gene ID (NCBI) | 6218 |
| RRID | AB_3671434 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08708 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPS17 is a component of the small ribosomal subunit, the ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS17 antibody 83848-4-RR | Download protocol |
| IF protocol for RPS17 antibody 83848-4-RR | Download protocol |
| FC protocol for RPS17 antibody 83848-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |