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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS17 Recombinant monoclonal antibody (83848-4-RR)

    WB analysis using 83848-4-RR (same clone as 83848-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83848-4-RR (RPS17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83848-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RPS17 Recombinant monoclonal antibody (83848-4-RR)

    IF Staining of U2OS using 83848-4-RR (same clone as 83848-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RPS17 antibody (83848-4-RR, Clone: 240851H3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83848-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using RPS17 Recombinant monoclonal antibody (83848-4-RR)

    FC experiment of HeLa using 83848-4-RR (same clone as 83848-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RPS17 Recombinant antibody (83848-4-RR, Clone:240851H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83848-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83848-4-RR

    Affinity and Kinetic Characterization of 83848-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83848-4-RR against Human RPS17 were performed. The affinity constant is 21.2 pM.

RPS17 Recombinant monoclonal antibody, PBS Only

RPS17 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83848-4-PBS
Clone No.240851H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

240851H3, 40S ribosomal protein S17, DBA4, ribosomal protein S17, RPS17L

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83848-4-PBS targets RPS17 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9334

Product name: Recombinant human RPS17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-135 aa of BC009407

Sequence: MGRVRTKTVKKAARVIIEKYYTRLGNDFHTNKRVCEEIAIIPSKKLRNKIAGYVTHLMKRIQRGPVRGISIKLQEEERERRDNYVPEVSALDQEIIEVDPDTKEMLKLLDFGSLSNLQVTQPTVGMNFKTPRGPV

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S17
Calculated molecular weight 135 aa, 16 kDa
Observed molecular weight16 kDa
GenBank accession numberBC009407
Gene Symbol RPS17
Gene ID (NCBI) 6218
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP08708
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RPS17 is a component of the small ribosomal subunit, the ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

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