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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS17 Polyclonal antibody (16267-1-AP)

    WB analysis using 16267-1-AP (same clone as 16267-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16267-1-AP (RPS17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16267-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RPS17 Polyclonal antibody (16267-1-AP)

    IHC staining of mouse cerebellum using 16267-1-AP (same clone as 16267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16267-1-AP (RPS17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16267-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RPS17 Polyclonal antibody (16267-1-AP)

    IHC staining of mouse cerebellum using 16267-1-AP (same clone as 16267-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16267-1-AP (RPS17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16267-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RPS17 Polyclonal antibody (16267-1-AP)

    IF Staining of U2OS using 16267-1-AP (same clone as 16267-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RPS17 antibody (16267-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16267-1-PBS in a different storage buffer formulation.

RPS17 Polyclonal antibody, PBS Only

RPS17 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16267-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16267-1-PBS targets RPS17 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9334

Product name: Recombinant human RPS17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-135 aa of BC009407

Sequence: MGRVRTKTVKKAARVIIEKYYTRLGNDFHTNKRVCEEIAIIPSKKLRNKIAGYVTHLMKRIQRGPVRGISIKLQEEERERRDNYVPEVSALDQEIIEVDPDTKEMLKLLDFGSLSNLQVTQPTVGMNFKTPRGPV

相同性解析による交差性が予測される生物種
Full Name ribosomal protein S17
Calculated molecular weight 135 aa, 16 kDa
Observed molecular weight16 kDa
GenBank accession numberBC009407
Gene Symbol RPS17
Gene ID (NCBI) 6218
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP08708
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RPS17 is a component of the small ribosomal subunit, the ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

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