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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS17 Polyclonal antibody (16267-1-AP)

    WB analysis using 16267-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16267-1-AP (RPS17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RPS17 Polyclonal antibody (16267-1-AP)

    IHC staining of mouse cerebellum using 16267-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16267-1-AP (RPS17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RPS17 Polyclonal antibody (16267-1-AP)

    IHC staining of mouse cerebellum using 16267-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 16267-1-AP (RPS17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RPS17 Polyclonal antibody (16267-1-AP)

    IF Staining of U2OS using 16267-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RPS17 antibody (16267-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

RPS17 Polyclonal antibody

RPS17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16267-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RPS17L2, RPS17L1, RPS17L, ribosomal protein S17, DBA4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, PC-3 cells, NIH/3T3 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16267-1-AP targets RPS17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS17 fusion protein Ag9334 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S17
Calculated molecular weight 135 aa, 16 kDa
Observed molecular weight16 kDa
GenBank accession numberBC009407
Gene Symbol RPS17
Gene ID (NCBI) 6218
RRIDAB_3085483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08708
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS17 is a component of the small ribosomal subunit, the ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

Protocols

Product Specific Protocols
WB protocol for RPS17 antibody 16267-1-APDownload protocol
IHC protocol for RPS17 antibody 16267-1-APDownload protocol
IF protocol for RPS17 antibody 16267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols