Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-83481-5 targets RPP30 in IF/ICC applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag8600 Product name: Recombinant human RPP30 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-268 aa of BC006991 Sequence: MAVFADLDLRAGSDLKALRGLVETAAHLGYSVVAINHIVDFKEKKQEIEKPVAVSELFTTLPIVQGKSRPIKILTRLTIIVSDPSHCNVLRATSSRARLYDVVAVFPKTEKLFHIACTHLDVDLVCITVTEKLPFYFKRPPINVAIDRGLAFELVYSPAIKDSTMRRYTISSALNLMQICKGKNVIISSAAERPLEIRGPYDVANLGLLFGLSESDAKAAVSTNCRAALLHGETRKTAFGIISTVKKPRPSEGDEDCLPASKKAKCEG 相同性解析による交差性が予測される生物種 |
| Full Name | ribonuclease P/MRP 30kDa subunit |
| Calculated molecular weight | 268 aa, 29 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC006991 |
| Gene Symbol | RPP30 |
| Gene ID (NCBI) | 10556 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P78346 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPP30 is a part of multimeric ribonuclease P complex and ribonuclease MRP complex. It is also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RPP30 antibody CL488-83481-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |