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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RPL7 Polyclonal antibody (14583-1-AP)

    WB analysis of HEK-293 using 14583-1-AP (same clone as 14583-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RPL7 Polyclonal antibody (14583-1-AP)

    WB analysis of HEK-293 using 14583-1-AP (same clone as 14583-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using RPL7 Polyclonal antibody (14583-1-AP)

    WB analysis of mouse kidney using 14583-1-AP (same clone as 14583-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using RPL7 Polyclonal antibody (14583-1-AP)

    IP experiment of HEK-293 using 14583-1-AP (same clone as 14583-1-PBS)

    IP result of anti-RPL7 (IP:14583-1-AP, 3ug; Detection:14583-1-AP 1:1000) with HEK-293 cells lysate 880ug. This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using RPL7 Polyclonal antibody (14583-1-AP)

    IHC staining of human skin cancer using 14583-1-AP (same clone as 14583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 14583-1-AP (RPL7 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using RPL7 Polyclonal antibody (14583-1-AP)

    IHC staining of human skin cancer using 14583-1-AP (same clone as 14583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 14583-1-AP (RPL7 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RPL7 Polyclonal antibody (14583-1-AP)

    IF Staining of HEK-293 using 14583-1-AP (same clone as 14583-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 14583-1-AP (RPL7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

RPL7 Polyclonal antibody, PBS Only

RPL7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14583-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14583-1-PBS targets RPL7 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6104

Product name: Recombinant human RPL7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-248 aa of BC006095

Sequence: MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN

相同性解析による交差性が予測される生物種
Full Name ribosomal protein L7
Calculated molecular weight 29 kDa
Observed molecular weight 29-30 kDa
GenBank accession numberBC006095
Gene Symbol RPL7
Gene ID (NCBI) 6129
RRIDAB_2254049
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP18124
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL7 binds to G-rich structures in 28S rRNA, and plays a regulatory role in the translation apparatus, inhibits cell-free translation of mRNAs. In addition, RPL7 is suggested to have a role as an integral part of natural freeze tolerance[PMID: 18706984]. Besides, RPL7 is responsible for its normal ribosome functions including the nuclear targeting, ribosome assembly and translation[PMID:17258209].

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