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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL7 Polyclonal antibody

RPL7 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14583-1-AP

Synonyms

60S ribosomal protein L7, humL7 1, ribosomal protein L7, RPL7

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 14583-1-AP

    WB analysis of HEK-293 using 14583-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 14583-1-AP

    WB analysis of mouse kidney using 14583-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 14583-1-AP

    WB analysis of HEK-293 using 14583-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 14583-1-AP

    IP experiment of HEK-293 using 14583-1-AP

    IP result of anti-RPL7 (IP:14583-1-AP, 3ug; Detection:14583-1-AP 1:1000) with HEK-293 cells lysate 880ug.

  • IHC staining of human skin cancer using 14583-1-AP

    IHC staining of human skin cancer using 14583-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 14583-1-AP (RPL7 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin cancer using 14583-1-AP

    IHC staining of human skin cancer using 14583-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 14583-1-AP (RPL7 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HEK-293 using 14583-1-AP

    IF Staining of HEK-293 using 14583-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 14583-1-AP (RPL7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14583-1-AP targets RPL7 in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL7 fusion protein Ag6104 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L7
Calculated molecular weight 29 kDa
Observed molecular weight 29-30 kDa
GenBank accession numberBC006095
Gene symbol RPL7
Gene ID (NCBI) 6129
RRIDAB_2254049
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL7 binds to G-rich structures in 28S rRNA, and plays a regulatory role in the translation apparatus, inhibits cell-free translation of mRNAs. In addition, RPL7 is suggested to have a role as an integral part of natural freeze tolerance[PMID: 18706984]. Besides, RPL7 is responsible for its normal ribosome functions including the nuclear targeting, ribosome assembly and translation[PMID:17258209].

Protocols

Product Specific Protocols
WB protocol for RPL7 antibody 14583-1-APDownload protocol
IHC protocol for RPL7 antibody 14583-1-APDownload protocol
IF protocol for RPL7 antibody 14583-1-APDownload protocol
IP protocol for RPL7 antibody 14583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nat Cell Biol

METTL16 exerts an m6A-independent function to facilitate translation and tumorigenesis.

Authors - Rui Su
View Article
humanIHC

Proc Natl Acad Sci U S A

Local translation in nuclear condensate amyloid bodies.

Authors - Phaedra R Theodoridis
View Article
humanWB,RIP

Biochem Biophys Res Commun

CRISPR-based RNA-binding protein mapping in live cells.

Authors - Baiwen Chen
View Article
humanWB

Front Endocrinol (Lausanne)

Glucose controls co-translation of structurally related mRNAs via the mTOR and eIF2 pathways in human pancreatic beta cells

Authors - Manuel Bulfoni
View Article
humanWB

Science

Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function

Authors - Wonyul Jang
View Article
humanWB

Cell

QKI shuttles internal m7G-modified transcripts into stress granules and modulates mRNA metabolism

Authors - Zhicong Zhao
View Article