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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL10 Polyclonal antibody (17013-1-AP)

    WB analysis using 17013-1-AP (same clone as 17013-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17013-1-AP (RPL10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RPL10 Polyclonal antibody (17013-1-AP)

    WB analysis using 17013-1-AP (same clone as 17013-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17013-1-AP (RPL10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RPL10 Polyclonal antibody (17013-1-AP)

    IHC staining of mouse brain using 17013-1-AP (same clone as 17013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RPL10 Polyclonal antibody (17013-1-AP)

    IHC staining of mouse brain using 17013-1-AP (same clone as 17013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using RPL10 Polyclonal antibody (17013-1-AP)

    IHC staining of human liver using 17013-1-AP (same clone as 17013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using RPL10 Polyclonal antibody (17013-1-AP)

    IHC staining of human liver using 17013-1-AP (same clone as 17013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17013-1-PBS in a different storage buffer formulation.

RPL10 Polyclonal antibody, PBS Only

RPL10 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17013-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17013-1-PBS targets RPL10 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9969

Product name: Recombinant human RPL10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-214 aa of BC003358

Sequence: MGRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLCGHMVSDEYEQLSSEALEAARICANKYMVKSCGKDGFHIRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNKEHVIEALRRAKFKFPGRQKIHISKKWGFTKFNADEFEDMVAEKRLIPDGCGVKYIPSRGPLDKWRALHS

相同性解析による交差性が予測される生物種
Full Name ribosomal protein L10
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC003358
Gene Symbol RPL10
Gene ID (NCBI) 6134
RRIDAB_2181278
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP27635
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RPL10, also named as Large ribosomal subunit protein uL16, is a 214 amino acid protein, which belongs to the universal ribosomal protein uL16 family. RPL10 is a component of the large ribosomal subunit and is down-regulated during adipocyte, kidney, and heart differentiation. RPL10 shares 98% amino acid sequence with RPL10L, so RPL10 antibody is able to recognize RPL10 and RPL10L protein.

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