Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, COLO 320 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| RIP | See 1 publications below |
Product Information
17013-1-AP targets RPL10 in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9969 Product name: Recombinant human RPL10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-214 aa of BC003358 Sequence: MGRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLCGHMVSDEYEQLSSEALEAARICANKYMVKSCGKDGFHIRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNKEHVIEALRRAKFKFPGRQKIHISKKWGFTKFNADEFEDMVAEKRLIPDGCGVKYIPSRGPLDKWRALHS 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein L10 |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC003358 |
| Gene Symbol | RPL10 |
| Gene ID (NCBI) | 6134 |
| RRID | AB_2181278 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27635 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPL10, also named as Large ribosomal subunit protein uL16, is a 214 amino acid protein, which belongs to the universal ribosomal protein uL16 family. RPL10 is a component of the large ribosomal subunit and is down-regulated during adipocyte, kidney, and heart differentiation. RPL10 shares 98% amino acid sequence with RPL10L, so RPL10 antibody is able to recognize RPL10 and RPL10L protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RPL10 antibody 17013-1-AP | Download protocol |
| WB protocol for RPL10 antibody 17013-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Transl Oncol Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics. | ||
Development Lin28-mediated promotion of protein synthesis is critical for neural progenitor cell maintenance and brain development in mice. | ||
Brain GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation | ||
Cardiovasc Res The translational landscape of human vascular smooth muscle cells identifies novel short ORF-encoded peptide regulators for phenotype alteration | ||
EMBO Mol Med Portimine A toxin causes skin inflammation through ZAKα-dependent NLRP1 inflammasome activation |
