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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RPE65 Polyclonal antibody

RPE65 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17939

Synonyms

LCA2, mRPE65, rd12, Retinoid isomerohydrolase, Retinol isomerase, RP20, RPE65, sRPE65

Validation Data Gallery

Applications:
  • IF Staining of mouse eye using CL488-17939

    IF Staining of mouse eye using CL488-17939

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CoraLite® Plus 488 RPE65 antibody (CL488-17939) at dilution of 1:200.

Tested Applications

Positive IF detected inmouse eye tissue
For other applications, we recommend the unconjugated version of this antibody, 17939-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-17939 targets RPE65 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPE65 fusion protein Ag12356 相同性解析による交差性が予測される生物種
Full Name retinal pigment epithelium-specific protein 65kDa
Calculated molecular weight 533 aa, 61 kDa
Observed molecular weight 65 kDa, 61 kDa
GenBank accession numberBC075036
Gene symbol RPE65
Gene ID (NCBI) 6121
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPE65(Retinal pigment epithelium-specific 65 kDa protein) is also named as retinoid isomerohydrolase with the calculated molecular weight og 61 kDa and belongs to the carotenoid oxygenase family. It is a microsomal protein with isomerase activity in the retinoid visual cycle that playing a role in the isomerisation of all-trans (vitamin A) to 11-cis retinal, the chromophore of the visual pigments. It also might be involved in retinoid uptake of keratinocytes.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RPE65 antibody CL488-17939Download protocol
Standard Protocols
Click here to view our Standard Protocols