Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, MCF-7 cells, HEK-293T cells, RAW 264.7 cells, C2C12 cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
86740-1-RR targets RNF43 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg6490 Product name: Recombinant Human RNF43 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 24-197 aa of NM_001305544.1 Sequence: GFGRTGLVLAAAVESERSAEQKAIIRVIPLKMDPTGKLNLTLEGVFAGVAEITPAEGKLMQSHPLYLCNASDDDNLEPGFISIVKLESPRRAPRPCLSLASKARMAGERGASAVLFDITEDRAAAEQLQQPLGLTWPVVLIWGNDAEKLMEFVYKNQKAHVRIELKEPPAWPDY 相同性解析による交差性が予測される生物種 |
| Full Name | ring finger protein 43 |
| Calculated molecular weight | 86 kDa |
| Observed molecular weight | 86 kDa |
| GenBank accession number | NM_001305544.1 |
| Gene Symbol | RNF43 |
| Gene ID (NCBI) | 54894 |
| RRID | AB_3745094 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q68DV7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RNF43 antibody 86740-1-RR | Download protocol |
| WB protocol for RNF43 antibody 86740-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |