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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF145 Polyclonal antibody (24524-1-AP)

    WB analysis using 24524-1-AP (same clone as 24524-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24524-1-AP (RNF145 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24524-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RNF145 Polyclonal antibody (24524-1-AP)

    WB analysis using 24524-1-AP (same clone as 24524-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24524-1-AP (RNF145 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24524-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RNF145 Polyclonal antibody (24524-1-AP)

    IHC staining of human lung cancer using 24524-1-AP (same clone as 24524-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24524-1-AP (RNF145 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24524-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RNF145 Polyclonal antibody (24524-1-AP)

    IHC staining of human lung cancer using 24524-1-AP (same clone as 24524-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24524-1-AP (RNF145 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24524-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RNF145 Polyclonal antibody, PBS Only

RNF145 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24524-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:2.3.2.27, RING finger protein 145

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24524-1-PBS targets RNF145 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21463

Product name: Recombinant human RNF145 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 492-663 aa of BC042684

Sequence: IHSYYNVWLRAQLGWKSFLLRRDAVNKIKSLPIATKEQLEKHNDICAICYQDMKSAVITPCSHFFHAGCLKKWLYVQETCPLCHCHLKNSSQLPGLGTEPVLQPHAGAEQNVMFQEGTEPPGQEHTPGTRIQEGSRDNNEYIARRPDNQEGAFDPKEYPHSAKDEVHPVESA

相同性解析による交差性が予測される生物種
Full Name ring finger protein 145
Calculated molecular weight 663 aa, 76 kDa
Observed molecular weight 76-79 kDa
GenBank accession numberBC042684
Gene Symbol RNF145
Gene ID (NCBI) 153830
RRIDAB_2879589
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MT1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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