Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, mouse kidney tissue, HepG2 cells, rat kidney tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IP | See 2 publications below |
Product Information
24524-1-AP targets RNF145 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RNF145 fusion protein Ag21463 相同性解析による交差性が予測される生物種 |
| Full Name | ring finger protein 145 |
| Calculated molecular weight | 663 aa, 76 kDa |
| Observed molecular weight | 76-79 kDa |
| GenBank accession number | BC042684 |
| Gene Symbol | RNF145 |
| Gene ID (NCBI) | 153830 |
| RRID | AB_2879589 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96MT1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF145 antibody 24524-1-AP | Download protocol |
| IHC protocol for RNF145 antibody 24524-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. | ||
EMBO J Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2
| ||
World J Surg Oncol Hypoxia-based classification and prognostic signature for clinical management of hepatocellular carcinoma | ||
J Agric Food Chem Methionine and Leucine Promote mTOR Gene Transcription and Milk Synthesis in Mammary Epithelial Cells through the eEF1Bα-UBR5-ARID1A Signaling |