Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, fetal human brain tissue, PC-3 cells |
Positive IF/ICC detected in | K562 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
86019-1-RR targets RNF10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | RNF10 fusion protein Ag10433 相同性解析による交差性が予測される生物種 |
Full Name | ring finger protein 10 |
Calculated molecular weight | 811 aa, 90 kDa |
Observed molecular weight | 90 kDa |
GenBank accession number | BC016622 |
Gene Symbol | RNF10 |
Gene ID (NCBI) | 9921 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q8N5U6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. RNF10 (RING finger protein 10), a 90-kDa protein, contains a ring finger motif which is known to be involved in protein-protein interactions. RNF10 regulates myelin-associated glycoprotein (MAG) expression and is required for myelin formation, therefore, participates in the peripheral nerve development and Schawnn cell differentiation. RNF10 is also involved in activation of p21WAF1 promoter via its activity as transcriptional factor.
Protocols
Product Specific Protocols | |
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WB protocol for RNF10 antibody 86019-1-RR | Download protocol |
IF protocol for RNF10 antibody 86019-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |