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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using RNF10 Recombinant antibody (86019-1-RR)

    WB analysis of Jurkat using 86019-1-RR (same clone as 86019-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 86019-1-RR (RNF10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using RNF10 Recombinant antibody (86019-1-RR)

    WB analysis of fetal human brain using 86019-1-RR (same clone as 86019-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 86019-1-RR (RNF10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using RNF10 Recombinant antibody (86019-1-RR)

    WB analysis of PC-3 using 86019-1-RR (same clone as 86019-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 86019-1-RR (RNF10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K562 using RNF10 Recombinant antibody (86019-1-RR)

    IF Staining of K562 using 86019-1-RR (same clone as 86019-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K562 using RNF10 antibody (86019-1-RR, Clone: 250438C11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

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RNF10 Recombinant antibody, PBS Only

RNF10 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86019-1-PBS
Clone No.250438C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase RNF10, EC:2.3.2.27, KIAA0262, RIE2, RING finger protein 10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86019-1-PBS targets RNF10 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNF10 fusion protein Ag10433 相同性解析による交差性が予測される生物種
Full Name ring finger protein 10
Calculated molecular weight 811 aa, 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC016622
Gene Symbol RNF10
Gene ID (NCBI) 9921
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N5U6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. RNF10 (RING finger protein 10), a 90-kDa protein, contains a ring finger motif which is known to be involved in protein-protein interactions. RNF10 regulates myelin-associated glycoprotein (MAG) expression and is required for myelin formation, therefore, participates in the peripheral nerve development and Schawnn cell differentiation. RNF10 is also involved in activation of p21WAF1 promoter via its activity as transcriptional factor.