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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated RIF1 Polyclonal anti (CL488-30119)

    IF Staining of HEK-293 using CL488-30119

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 RIF1 antibody (CL488-30119) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated RIF1 Polyclonal anti (CL488-30119)

    IF Staining of HeLa using CL488-30119

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RIF1 antibody (CL488-30119) at dilution of 1:200.

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CoraLite® Plus 488-conjugated RIF1 Polyclonal antibody

RIF1 Polyclonal Antibody for IF/ICC
Cat No. CL488-30119

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC

Synonyms

DKFZp434D1026, DKFZp781N1478, FLJ12870, RIF1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-30119 targets RIF1 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIF1 fusion protein Ag32422 相同性解析による交差性が予測される生物種
Full Name RAP1 interacting factor homolog (yeast)
Calculated molecular weight274 kDa
Observed molecular weight300 kDa
GenBank accession numberNM_001177663.1
Gene Symbol RIF1
Gene ID (NCBI) 55183
RRIDAB_3084125
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5UIP0
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RIF1 (Rap1-interacting factor 1) is a multifunctional chromosome stability protein implicated in controlling DNA replication and repair. Human RIF1 can interact with protein phosphatase 1 (PP1) and the interaction is crucial for controlling DNA replication by limiting phosphorylation of the MCM complex (PMID: 28077461). RIF1 also acts with PP1 to protect nascent DNA from overdegradation at stalled replication forks (PMID: 31141682). RIF1 promotes non-homologous end joining (NHEJ) in double strand break (DSB) repair (PMID: 30443189). Research studies show that RIF1 is overexpressed in breast cancer tissues and is upgraded in NSCLC tissues (PMID: 30237512).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RIF1 antibody CL488-30119Download protocol
Standard Protocols
Click here to view our Standard Protocols