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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RIC8A Monoclonal antibody

RIC8A Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E3B11

Cat no : CL488-66625

Synonyms

Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A

Validation Data Gallery

Applications:
  • IF Staining of mouse brain using CL488-66625

    IF Staining of mouse brain using CL488-66625

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 RIC8A antibody (CL488-66625, Clone: 1E3B11 ) at dilution of 1:200.

  • IF Staining of mouse brain using CL488-66625

    IF Staining of mouse brain using CL488-66625

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 RIC8A antibody (CL488-66625, Clone: 1E3B11 ) at dilution of 1:200.

Tested Applications

Positive IF detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 66625-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66625 targets RIC8A in IF applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RIC8A fusion protein Ag1593 相同性解析による交差性が予測される生物種
Full Name resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
Calculated molecular weight 60 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC011821
Gene symbol RIC8A
Gene ID (NCBI) 60626
RRIDAB_2919352
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RIC8A antibody CL488-66625Download protocol
Standard Protocols
Click here to view our Standard Protocols