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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RGS9BP Polyclonal antibody (33040-1-AP)

    WB analysis using 33040-1-AP (same clone as 33040-1-PBS)

    Mouse eye tissue lysate was subjected to SDS PAGE followed by western blot with 33040-1-AP (RGS9BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RGS9BP Polyclonal antibody (33040-1-AP)

    IHC staining of mouse eye using 33040-1-AP (same clone as 33040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 33040-1-AP (RGS9BP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RGS9BP Polyclonal antibody (33040-1-AP)

    IHC staining of mouse eye using 33040-1-AP (same clone as 33040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 33040-1-AP (RGS9BP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat eye tissue using RGS9BP Polyclonal antibody (33040-1-AP)

    IHC staining of rat eye using 33040-1-AP (same clone as 33040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 33040-1-AP (RGS9BP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat eye tissue using RGS9BP Polyclonal antibody (33040-1-AP)

    IHC staining of rat eye using 33040-1-AP (same clone as 33040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 33040-1-AP (RGS9BP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33040-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using RGS9BP Polyclonal antibody (33040-1-AP)

    IF Staining of mouse eye using 33040-1-AP (same clone as 33040-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RGS9BP antibody (33040-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33040-1-PBS in a different storage buffer formulation.

RGS9BP Polyclonal antibody, PBS Only

RGS9BP Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 33040-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

R9AP, Regulator of G-protein signaling 9-binding protein, RGS9-anchoring protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

33040-1-PBS targets RGS9BP in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag37833

Product name: Recombinant human RGS9BP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-210 aa of NM_207391

Sequence: MAREECKALLDGLNKTTACYHHLVLTVGGSADSQNLRQELQKTRQKAQELAVSTCARLTAVLRDRGLAADERAEFERLWVAFSGCLDLLEADMRRALELGAAFPLHAPRRPLVRTGVAGASSGVAARALSTRSLRLEAEGDFDVADLRELEREVLQVGEMIDNMEMKVNVPRWTVQARQAAGAELLSTVSAGPSSVVSLQERGGGCDPRK

相同性解析による交差性が予測される生物種
Full Name regulator of G protein signaling 9 binding protein
Calculated molecular weight235aa, 25 kDa
Observed molecular weight30 kDa
GenBank accession numberNM_207391
Gene Symbol RGS9BP
Gene ID (NCBI) 388531
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ6ZS82
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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