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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RGS9BP Polyclonal antibody (33040-1-AP)

    WB analysis using 33040-1-AP

    Mouse eye tissue lysate was subjected to SDS PAGE followed by western blot with 33040-1-AP (RGS9BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RGS9BP Polyclonal antibody (33040-1-AP)

    IHC staining of mouse eye using 33040-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 33040-1-AP (RGS9BP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RGS9BP Polyclonal antibody (33040-1-AP)

    IHC staining of mouse eye using 33040-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 33040-1-AP (RGS9BP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat eye tissue using RGS9BP Polyclonal antibody (33040-1-AP)

    IHC staining of rat eye using 33040-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 33040-1-AP (RGS9BP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat eye tissue using RGS9BP Polyclonal antibody (33040-1-AP)

    IHC staining of rat eye using 33040-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 33040-1-AP (RGS9BP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using RGS9BP Polyclonal antibody (33040-1-AP)

    IF Staining of mouse eye using 33040-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RGS9BP antibody (33040-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RGS9BP Polyclonal antibody

RGS9BP Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 33040-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

R9AP, Regulator of G-protein signaling 9-binding protein, RGS9-anchoring protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse eye tissue
Positive IHC detected inmouse eye tissue, rat eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

33040-1-AP targets RGS9BP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag37833

Product name: Recombinant human RGS9BP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-210 aa of NM_207391

Sequence: MAREECKALLDGLNKTTACYHHLVLTVGGSADSQNLRQELQKTRQKAQELAVSTCARLTAVLRDRGLAADERAEFERLWVAFSGCLDLLEADMRRALELGAAFPLHAPRRPLVRTGVAGASSGVAARALSTRSLRLEAEGDFDVADLRELEREVLQVGEMIDNMEMKVNVPRWTVQARQAAGAELLSTVSAGPSSVVSLQERGGGCDPRK

相同性解析による交差性が予測される生物種
Full Name regulator of G protein signaling 9 binding protein
Calculated molecular weight235aa, 25 kDa
Observed molecular weight30 kDa
GenBank accession numberNM_207391
Gene Symbol RGS9BP
Gene ID (NCBI) 388531
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ6ZS82
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for RGS9BP antibody 33040-1-APDownload protocol
IHC protocol for RGS9BP antibody 33040-1-APDownload protocol
WB protocol for RGS9BP antibody 33040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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