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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RGS12 Polyclonal antibody (29803-1-AP)

    WB analysis using 29803-1-AP (same clone as 29803-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RGS12 Polyclonal antibody (29803-1-AP)

    WB analysis using 29803-1-AP (same clone as 29803-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using RGS12 Polyclonal antibody (29803-1-AP)

    IHC staining of rat cerebellum using 29803-1-AP (same clone as 29803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29803-1-AP (RGS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using RGS12 Polyclonal antibody (29803-1-AP)

    IF Staining of PC-3 using 29803-1-AP (same clone as 29803-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using RGS12 antibody (29803-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

RGS12 Polyclonal antibody, PBS Only

RGS12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29803-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Regulator of G-protein signaling 12

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29803-1-PBS targets RGS12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30731

Product name: Recombinant human RGS12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 833-962 aa of NM_198229

Sequence: LAEVEGRALPDSQQVPSSPASKHSLGSDHSSVSTPKKLSGKSKSGRSLNEELGDEDSEKKRKGAFFSWSRTRSTGRSQKKREHGDHADDALHANGGLCRRESQGSVSSAGSLDLSEACRTLAPEKDKATK

相同性解析による交差性が予測される生物種
Full Name regulator of G-protein signaling 12
Calculated molecular weight 156 kDa
Observed molecular weight140-150 kDa
GenBank accession numberNM_198229
Gene Symbol RGS12
Gene ID (NCBI) 6002
RRIDAB_2935482
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14924
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Regulator of G-protein signaling 12 (RGS12) regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. This antibody can be detected as 140-150 kDa.

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