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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RGS12 Polyclonal antibody

RGS12 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 29803-1-AP

Synonyms

DKFZp761K1617, DKFZp761K1817, RGS12

Validation Data Gallery

Applications:
  • WB analysis using 29803-1-AP

    WB analysis using 29803-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29803-1-AP

    WB analysis using 29803-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 29803-1-AP

    IF Staining of PC-3 using 29803-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using RGS12 antibody (29803-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, A431 cells, SH-SY5Y cells, THP-1 cells, NIH/3T3 cells
Positive IF detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy RGS12 Ready-To-Use IHC Kit. RGS12 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

29803-1-AP targets RGS12 in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RGS12 fusion protein Ag30731 相同性解析による交差性が予測される生物種
Full Name regulator of G-protein signaling 12
Calculated molecular weight 156 kDa
Observed molecular weight140-150 kDa
GenBank accession numberNM_198229
Gene symbol RGS12
Gene ID (NCBI) 6002
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Regulator of G-protein signaling 12 (RGS12) regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. This antibody can be detected as 140-150 kDa.

Protocols

Product Specific Protocols
WB protocol for RGS12 antibody 29803-1-APDownload protocol
IF protocol for RGS12 antibody 29803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols