Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, A431 cells, SH-SY5Y cells, THP-1 cells, NIH/3T3 cells |
| Positive IHC detected in | rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29803-1-AP targets RGS12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30731 Product name: Recombinant human RGS12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 833-962 aa of NM_198229 Sequence: LAEVEGRALPDSQQVPSSPASKHSLGSDHSSVSTPKKLSGKSKSGRSLNEELGDEDSEKKRKGAFFSWSRTRSTGRSQKKREHGDHADDALHANGGLCRRESQGSVSSAGSLDLSEACRTLAPEKDKATK 相同性解析による交差性が予測される生物種 |
| Full Name | regulator of G-protein signaling 12 |
| Calculated molecular weight | 156 kDa |
| Observed molecular weight | 140-150 kDa |
| GenBank accession number | NM_198229 |
| Gene Symbol | RGS12 |
| Gene ID (NCBI) | 6002 |
| RRID | AB_2935482 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14924 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Regulator of G-protein signaling 12 (RGS12) regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. This antibody can be detected as 140-150 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RGS12 antibody 29803-1-AP | Download protocol |
| WB protocol for RGS12 antibody 29803-1-AP | Download protocol |
| IF protocol for RGS12 antibody 29803-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |